Cold cataract is a phase separation effect that proceeds via spinodal decomposition of the lens cytoplasm with cooling. We present a detailed dynamic light scattering study on the phase separation in the ocular lens emerging during cold cataract development. Box 1414, GR–26504, Patras, GreeceĢ Department of Chemical Engineering, University of Patras, GR–26504, Patras, Greece 3 Medical School, Department of Ophthalmology, University of Patras, GR–26504, Patras, Greece Yannopoulos1,*ġ Foundation for Research and Technology Hellas – Institute of Chemical Engineering and High Temperature Chemical Processes (FORTH / ICE-HT), P.O. By contrast, DLS looks at size based on the diffusion of particles in solution, so DLS needs a bit of time to detect particles that are moving around.īut on the plus side, DLS is more quantitative when you know less about your sample, so it’s great for comparing sizes from day-to-day or batch-to-batch, and for measuring how the size of your particles change over long isothermal experiments.Dynamic Light Scattering on Protein Aggregation: Study of Cold Cataractĭynamic light scattering study on phase separation of a protein-water mixture: Application on cold cataract development in the ocular lens So when you're thinking about choosing the right kind of light scattering, SLS is ideal for detecting the moment when aggregation begins because it is a direct measurement that increases the moment molecular weight increases. You can say it’s like listening to a rock concert-SLS would tell you how loud the music is and DLS would tell you what song is being played. In contrast, DLS reads how fast scattered light intensity changes over time, which provides information on diffusion rates and particle size. SLS is all about average intensity and can tell us about particle molecular weight and concentration in certain setups. Instruments often offer s tatic light scattering (SLS) and dynamic light scattering (DLS) data side-by-side, so you may be wondering about the differences between the techniques and when to use each one. Monoclonal antibodies & recombinant proteins.Ultrafiltration & Diafiltration (UF/DF).Differential Scanning Fluorimetry (DSF).The unrivaled particle characterization and ID platform Hound – The unrivaled particle characterization and ID platform.The all-around configurable benchtop workflow solution Junior – The all-around configurable benchtop workflow solution.The only completely hands-free benchtop buffer exchange solution Unagi – The only completely hands-free benchtop buffer exchange solution.The first all-in-one biologics stability screening platform Uncle – The first all-in-one biologics stability screening platform.Honeybun – The most rapid viscometer out there.The one of a kind silicone thickness and distribution analyzer Bouncer – The one of a kind silicone thickness and distribution analyzer.The most customizable automated workflow solution Big Kahuna – The most customizable automated workflow solution.The ultimate automated buffer exchange solution Big Tuna – The ultimate automated buffer exchange solution.The next-gen protein and nucleic acid quantification system Lunatic – The next-gen protein and nucleic acid quantification system.Stunner – The ultimate gene therapy tool.
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